Isolation of Active Ribosomal Subunits from Yeast

Preparation of Biologically Active Ribosomal Subunits from Fungal Spores

Preparation of biologically active ribosomal subunits from fungal spores.

Ribosomal subunits were prepared from fungal spore ribosomes and reassociated to yield biologically active ribosomes.

Structure and function of mammalian ribosomes. I. Isolation and characterization of active liver ribosomal subunits.

Wo have developed a method for preparation and purification of active ribosomal subunits from rat and mouse liver. Our technique involvea incubating purified polysomes with all components for protein synthesis until all competent ribosomes have terminated and released their polypeptide chain. Upon kreatment of such an incubation mixture with 0.5 M-KCl, followed by sucrose gradient centrifugatio...

Isolation of 30S and 50S active ribosomal subunits of Bacillus subtilis, Marburg strain.

Active 30S and 50S ribosomal subunits were isolated from Bacillus subtilis. These subunits were able to perform not only protein synthesis in the presence of artificial or natural messenger ribonucleic acid but also the specific functions characteristic of each of the subunits. Thus the 30S subunits alone are able to bind formyl-methionyl-transfer ribonucleic acid, and the 50S subunits carry th...

Isolation and characterization of mitochondrial ribosomes and ribosomal subunits from Leishmania tarentolae.

We have analyzed Leishmania tarentolae mitochondrial ribonucleoprotein (RNP) complexes using the 9S small subunit (SSU) rRNA and the 12S large subunit (LSU) rRNA as markers, and have identified a 50S RNP particle as the putative mitochondrial monosome, a 40S particle as the putative LSU and a 30S particle as the putative SSU. These assignments are supported by morphological analysis by cryo-ele...